Anti-Human CD44 Recombinant Antibody (RG7356)

Customer Review

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David Anderson
15.Jan,24

Ideal for Therapeutic Studies

The Anti-CD44 Therapeutic Antibody (RG7356) has been a fantastic addition to our therapeutic research. Its specificity and sensitivity are outstanding, consistently providing clear results. This antibody has become essential for our studies, improving the accuracy and reliability of our assays.

Jessica Hall
28.Jul,23

Great for Immunoassays

We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for CD44 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.

Laura Walker
02.Nov,22

Consistent and Effective

This antibody has proven to be consistent and effective in our various assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for CD44 have been particularly beneficial.

Q&As

1. Is the anti-CD44 therapeutic antibody RG7356 suitable for blocking CD44-mediated cell adhesion in vitro?

   A: Yes, the anti-CD44 therapeutic antibody RG7356 (TAB-128CL) is suitable for blocking CD44-mediated cell adhesion in vitro. It can effectively inhibit the interaction between CD44 and its ligands, which is useful for studying cell adhesion mechanisms.

2. What is the recommended storage condition for the anti-CD44 therapeutic antibody RG7356?

   A: The recommended storage condition for the anti-CD44 therapeutic antibody RG7356 (TAB-128CL) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-CD44 therapeutic antibody RG7356 be used in flow cytometry to analyze CD44 expression?

   A: Yes, the anti-CD44 therapeutic antibody RG7356 (TAB-128CL) can be used in flow cytometry to analyze CD44 expression. It is designed to specifically bind to CD44, making it suitable for such applications.

4. Is the anti-CD44 therapeutic antibody RG7356 effective in Western blotting?

   A: Yes, the anti-CD44 therapeutic antibody RG7356 (TAB-128CL) is effective in Western blotting. It provides specific binding to CD44, allowing for reliable detection in Western blot assays.

5. What is the optimal dilution for using the anti-CD44 therapeutic antibody RG7356 in immunofluorescence?

   A: The optimal dilution for using the anti-CD44 therapeutic antibody RG7356 (TAB-128CL) in immunofluorescence is typically 1:100 to 1:500. It is recommended to perform a dilution series to determine the best working concentration for specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Solier, Stéphanie, et al. "A druggable copper-signalling pathway that drives inflammation." Nature 617.7960 (2023): 386-394. https://doi.org/s41586-023-06017-4
   This study focuses on the role of CD44-mediated copper uptake in regulating inflammation through metabolic and epigenetic reprogramming in macrophages. The researchers found that CD44 facilitates the uptake of copper(II) in mitochondria, which catalyzes NAD(H) redox cycling, promoting metabolic changes and subsequent epigenetic modifications that lead to an inflammatory state. They developed a metformin dimer, LCC-12, which inactivates mitochondrial copper(II), thereby inducing metabolic and epigenetic shifts that counteract macrophage activation. This study highlights the potential of targeting mitochondrial copper(II) as a therapeutic strategy to reduce inflammation in various disease models, including bacterial and viral infections.
   Creative Biolabs contributed significantly to this research by providing the Anti-CD44 antibody (Cat# TAB-128CL). This antibody was crucial for the experiments involving the detection and analysis of CD44-mediated copper uptake in macrophages. The precise measurement and modulation of CD44 activity facilitated a deeper understanding of the mechanisms by which CD44 regulates macrophage metabolism and inflammation, thus supporting the study's exploration of new therapeutic approaches based on metabolic reprogramming.
2. Gomez, Karina E., et al. "Cancer cell CD44 mediates macrophage/monocyte-driven regulation of head and neck cancer stem cells." Cancer research 80.19 (2020): 4185-4198. https://doi.org/10.1158/0008-5472.CAN-20-1079
   This study explores the interaction between tumor-associated macrophages (TAMs) and cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC), focusing on the role of the CD44 receptor. The researchers found that TAMs enhance the stemness and invasive properties of CSCs by modulating CD44 signaling pathways, particularly through the PI3K-4EBP1-SOX2 axis. Using patient-derived samples, in vitro coculture spheroid models, and in vivo mouse models, the study demonstrated that TAMs increase the availability of hyaluronic acid (HA), which binds to CD44 and activates signaling pathways that promote CSC growth and invasion. This mechanistic link between TAMs and CSCs provides insights into potential therapeutic targets for HNSCC.
   Creative Biolabs provided essential reagents for this study, specifically the anti-CD44 monoclonal antibody (Cat# TAB-128CL). This antibody was crucial for the experiments involving CD44 detection and functional analysis in cancer cells. By enabling precise measurement and modulation of CD44 activity, the antibody facilitated a deeper understanding of how CD44 mediates the interaction between TAMs and CSCs. The use of Creative Biolabs' high-quality reagents significantly advanced the study's investigation into the molecular mechanisms underlying CSC regulation by TAMs.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# TAB-128CL, RRID: AB_3111826)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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